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Clinical and Vaccine Immunology, November 2006, p. 1185-1189, Vol. 13, No. 11
1071-412X/06/$08.00+0     doi:10.1128/CVI.00229-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Evaluation of an Enzyme Immunoassay for Detection of Dengue Virus NS1 Antigen in Human Serum

Centre National de Référence des Arbovirus, Région Antilles-Guyane, Institut Pasteur de la Guyane, Cayenne, French Guiana,¹ Laboratoire de Virologie-Immunologie, Centre Hospitalier Universitaire de Fort de France, Martinique, French West Indies,² Laboratoire de Biologie Clinique, Centre Hospitalier de la Basse Terre, Guadeloupe, French West Indies,³ Department of Arboviruses, Instituto Evandro Chagas Secretaria de Vigilância em Saude, Ministerio da Saude, Belém, Brazil,⁴ Laboratoire de Microbiologie, Centre Hospitalier Universitaire de Pointe à Pitre, Guadeloupe, French West Indies,⁵ Département de Virologie, Institut Pasteur, Paris, France,⁶ Unité d'Epidémiologie des Maladies Emergentes, Institut Pasteur, Paris, France⁷

Received 13 June 2006/ Returned for modification 24 July 2006/ Accepted 28 August 2006

We evaluated a one-step sandwich-format microplate enzyme immunoassay for detecting dengue virus NS1 antigen (Ag) in human serum by use of Platelia Dengue NS1 Ag kits (Bio-Rad Laboratories, Marnes La Coquette, France). We collected 299 serum samples from patients with dengue disease and 50 serum samples from patients not infected with dengue virus. For the 239 serum samples from patients with acute infections testing positive by reverse transcription-PCR and/or virus isolation for one of the four dengue virus serotypes, the sensitivity of the Platelia Dengue NS1 Ag kit was 88.7% (95% confidence interval, 84.0% to 92.4%). None of the serum samples from patients not infected with dengue virus tested positive with the Platelia Dengue NS1 Ag kit. A diagnostic strategy combining the Platelia Dengue NS1 Ag test for acute-phase sera and immunoglobulin M capture enzyme-linked immunosorbent assay for early-convalescent-phase sera increased sensitivity only from 88.7% to 91.9%. Thus, NS1 antigen detection with the Platelia Dengue NS1 Ag kit could be used for first-line testing for acute dengue virus infection in clinical diagnostic laboratories.

Published ahead of print on 20 September 2006.

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