This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Blacksell, S. D.
Right arrow Articles by Newton, P. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Blacksell, S. D.
Right arrow Articles by Newton, P. N.

 Previous Article  |  Next Article 

Clinical and Vaccine Immunology, October 2006, p. 1166-1169, Vol. 13, No. 10
1071-412X/06/$08.00+0     doi:10.1128/CVI.00219-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Limited Diagnostic Capacities of Two Commercial Assays for the Detection of Leptospira Immunoglobulin M Antibodies in Laos

Stuart D. Blacksell,1,2,3* Lee Smythe,4 Rattanaphone Phetsouvanh,1 Michael Dohnt,4 Rudy Hartskeerl,5 Meegan Symonds,4 Andrew Slack,4 Manivanh Vongsouvath,1 Viengmone Davong,1 Olay Lattana,1 Simmaly Phongmany,1 Valy Keolouangkot,1 Nicholas J. White,1,2,3 Nicholas P. J. Day,1,2,3 and Paul N. Newton1,2*

Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic,1 Centre for Clinical Vaccinology and Tropical Medicine, University of Oxford, Churchill Hospital, Oxford OX3 7LJ, United Kingdom,2 Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Rd., Bangkok 10400, Thailand,3 WHO/FAO/OIE Collaborating Centre for Reference and Research on Leptospirosis, Coopers Plains, Queensland, 4108, Australia,4 WHO/FAO/OIE Collaborating Centre for Reference and Research on Leptospirosis, KIT Biomedical Research, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands5

Received 2 June 2006/ Returned for modification 11 July 2006/ Accepted 31 July 2006

The diagnostic utility of immunochromatographic (Leptotek) and enzyme-linked immunosorbent assay (ELISA; Panbio) tests for the detection of Leptospira immunoglobulin M antibodies was assessed in febrile adults admitted in Vientiane, Laos. Both tests demonstrated poor diagnostic accuracy using admission serum (Leptotek sensitivity of 47.3% and specificity of 75.5%: ELISA sensitivity of 60.9% and specificity of 65.6%) compared to the Leptospira "gold standard" microscopic agglutination test.

* Corresponding authors. Mailing address for P. Newton: Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao PDR. Phone: (856) 21 242168. Fax: (856) 21 242168. E-mail: paul{at}tropmedres.ac. Mailing address for S. Blacksell: Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Rd., Bangkok 10400, Thailand. Phone: (66) 2 354 9171. Fax: (66) 2 354 9169. E-mail: stuart{at}tropmedres.ac.

Clinical and Vaccine Immunology, October 2006, p. 1166-1169, Vol. 13, No. 10
1071-412X/06/$08.00+0     doi:10.1128/CVI.00219-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lin, X., Chen, Y., Yan, J. (2008). Recombinant Multiepitope Protein for Diagnosis of Leptospirosis. CVI 15: 1711-1714 [Abstract] [Full Text]  
  • McBride, A. J. A., Santos, B. L., Queiroz, A., Santos, A. C., Hartskeerl, R. A., Reis, M. G., Ko, A. I. (2007). Evaluation of Four Whole-Cell Leptospira-Based Serological Tests for Diagnosis of Urban Leptospirosis. CVI 14: 1245-1248 [Abstract] [Full Text]  
  • Croda, J., Ramos, J. G. R., Matsunaga, J., Queiroz, A., Homma, A., Riley, L. W., Haake, D. A., Reis, M. G., Ko, A. I. (2007). Leptospira Immunoglobulin-Like Proteins as a Serodiagnostic Marker for Acute Leptospirosis. J. Clin. Microbiol. 45: 1528-1534 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»