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Clinical and Vaccine Immunology, October 2006, p. 1119-1124, Vol. 13, No. 10
1071-412X/06/$08.00+0     doi:10.1128/CVI.00209-06

Field Evaluation of a Novel Differential Diagnostic Reagent for Detection of Mycobacterium bovis in Cattle{triangledown}

P. J. Cockle,* S. V. Gordon, R. G. Hewinson, and H. M. Vordermeier

TB Research Group, Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone KT15 3NB, United Kingdom

Received 6 June 2006/ Returned for modification 7 July 2006/ Accepted 9 August 2006

In the search for improved tools with which to control bovine tuberculosis, the development of enhanced immunodiagnostic reagents is a high priority. Such reagents are required to improve the performance of tuberculin-based reagents and allow the discrimination of vaccinated cattle from those infected with Mycobacterium bovis. In this study, we identified the immunodominant, frequently recognized peptides from Rv3873, Rv3879c, Rv0288, and Rv3019c, which, together with peptides comprising the current lead diagnostic antigens, ESAT-6 and CFP-10, were formulated into a peptide cocktail. In a test of naturally infected cattle, this cocktail was significantly better than tuberculin was for identifying skin test-negative animals with confirmed bovine tuberculosis. In addition, the specificity of this cocktail was not compromised by Mycobacterium bovis BCG vaccination. In summary, our results prioritize this peptide-based, fully synthetic reagent for assessment in larger trials.

* Corresponding author. Mailing address: TB Research Group, Department of Statutory and Exotic Bacterial Diseases, Veterinary Laboratories Agency-Weybridge, New Haw, Addlestone KT15 3NB, United Kingdom. Phone: 44 1932 357 768. Fax: 44 1932 357 684. E-mail: p.cockle{at}vla.defra.gsi.gov.uk.

{triangledown} Published ahead of print on 30 August 2006.

Clinical and Vaccine Immunology, October 2006, p. 1119-1124, Vol. 13, No. 10
1071-412X/06/$08.00+0     doi:10.1128/CVI.00209-06






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