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Clinical and Vaccine Immunology, October 2006, p. 1087-1091, Vol. 13, No. 10
1071-412X/06/$08.00+0     doi:10.1128/CVI.00211-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Sequencing of the porB Gene: a Step toward a True Characterization of Neisseria meningitidis

R. Abad, B. Alcalá, C. Salcedo, R. Enríquez, M. J. Uría, P. Diez, and J. A. Vázquez*

Reference Laboratory for Neisseria, National Centre of Microbiology, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo Km2, 28220, Majadahonda, Madrid, Spain

Received 16 May 2006/ Returned for modification 7 July 2006/ Accepted 2 August 2006

Variations in class 2/3 (PorB) proteins form the basis for meningococcal serotyping. Antibodies against these proteins are bactericidal, making serotyping results useful not only for epidemiological surveillance of meningococcal disease but also for identifying potential vaccine components. A total of 20 to 60% of meningococcal B and C isolates from any given population are nontypeable (NT) using a panel of monoclonal antibodies. To analyze the mechanisms responsible for the nonserotypeability characteristic in Neisseria meningitidis, we (i) established the nucleotide sequences of porB gene in 146 meningococcal strains (95 not recognized by the serotyping panel), (ii) identified 18 new allelic variants of the porB gene, (iii) correlated allelic variants with serotypes, (iv) suggest the nontypeability characteristic in those 95 NT strains, and (v) reject the possibility of variation in the levels of PorB expression.

* Corresponding author. Mailing address: Reference Laboratory for Neisseria, National Centre of Microbiology, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo Km2, 28220, Majadahonda, Madrid, Spain. Phone: 34918223617. Fax: 34915097966. E-mail: jvazquez{at}isciii.es.

Clinical and Vaccine Immunology, October 2006, p. 1087-1091, Vol. 13, No. 10
1071-412X/06/$08.00+0     doi:10.1128/CVI.00211-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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