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Clinical and Vaccine Immunology, January 2006, p. 152-155, Vol. 13, No. 1
1071-412X/06/$08.00+0     doi:10.1128/CVI.13.1.152-155.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Ultrasensitive Human Immunodeficiency Virus Type 1 p24 Antigen Assay Modified for Use on Dried Whole-Blood Spots as a Reliable, Affordable Test for Infant Diagnosis

Janet C. Patton,1* Gayle G. Sherman,1,2,3 Ashraf H. Coovadia,1 Wendy S. Stevens,2,3 and Tammy M. Meyers1,4

Wits Paediatric HIV Unit, Wits Health Consortium,1 Departments of Molecular Medicine and Hematology,2 Paediatrics, University of the Witwatersrand, Johannesburg, South Africa,4 National Health Laboratory Service, South Africa3

Received 9 July 2005/ Returned for modification 31 August 2005/ Accepted 31 October 2005

The ultrasensitive human immunodeficiency virus (HIV) p24 antigen assay was modified for use on pediatric dried whole-blood spots on Whatman no. 1 filter paper. The modified assay was found to be reliable and accurate, making it an affordable tool for pediatric HIV diagnosis in developing countries.

* Corresponding author. Mailing address: Department Molecular Medicine and Hematology, Faculty of Health Sciences, University of the Witwatersrand, 7 York Rd., Parktown 2193, Johannesburg, South Africa. Phone: 27-11-4898496. Fax: 27-11-4845812. E-mail: janet.patton{at}nhls.ac.za.

Clinical and Vaccine Immunology, January 2006, p. 152-155, Vol. 13, No. 1
1071-412X/06/$08.00+0     doi:10.1128/CVI.13.1.152-155.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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Copyright © 2006 by the American Society for Microbiology. All rights reserved.