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Clinical and Diagnostic Laboratory Immunology, September 2005, p. 1127-1129, Vol. 12, No. 9
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.9.1127-1129.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison of the Antibodies in Lymphocyte Supernatant and Antibody-Secreting Cell Assays for Measuring Intestinal Mucosal Immune Response to a Novel Oral Typhoid Vaccine (M01ZH09)

B. D. Kirkpatrick,1* Matthew D. Bentley,2 Anette M. Thern,2 Catherine J. Larsson,1 Cassandra Ventrone,1 Meera V. Sreenivasan,1 and Lou Bourgeois3

University of Vermont College of Medicine, Department of Medicine, Unit of Infectious Diseases, Burlington, Vermont,1 Microscience Limited, Wokingham, Berkshire, United Kingdom,2 Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland3

Received 4 May 2005/ Returned for modification 27 May 2005/ Accepted 10 June 2005

Antibody-secreting cell (ASC) and antibodies in lymphocyte supernatant (ALS) assays are used to assess intestinal mucosal responses to enteric infections and vaccines. The ALS assay, performed on cell supernatants, may represent a convenient alternative to the more established ASC assay. The two methods, measuring immunoglobulin A to Salmonella enterica serovar Typhi lipopolysaccharide, were compared in volunteers vaccinated with a live-attenuated typhoid vaccine M01ZH09. The specificity of the ALS assay compared to the ASC assay was excellent (100%), as was sensitivity (82%). The ALS assay was less sensitive than the ASC assay at ≤42 spots/106 peripheral blood lymphocytes.

* Corresponding author. Mailing address: University of Vermont College of Medicine, Unit of Infectious Diseases, 110 Stafford Building, 95 Carrigan Lane, Burlington, VT 05405. Phone: (802) 656-5822. Fax: (802) 656-0881. E-mail: beth.kirkpatrick{at}uvm.edu.

Clinical and Diagnostic Laboratory Immunology, September 2005, p. 1127-1129, Vol. 12, No. 9
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.9.1127-1129.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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