This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Green, B. J.
Right arrow Articles by Tovey, E. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Green, B. J.
Right arrow Articles by Tovey, E. R.
Right arrowPubmed/NCBI databases
*Substance via MeSH
Medline Plus Health Information
*Fungal Infections

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, September 2005, p. 1114-1116, Vol. 12, No. 9
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.9.1114-1116.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Detection of Aerosolized Alternaria alternata Conidia, Hyphae, and Fragments by Using a Novel Double-Immunostaining Technique

Brett James Green,1,2 Detlef Schmechel,3 and Euan Roger Tovey1,2*

Department of Medicine, The University of Sydney, NSW, Australia,1 Woolcock Institute of Medical Research, Sydney, NSW, Australia,2 Health Effects Laboratory Division, Centers for Disease Control and Prevention, National Institute of Occupational Safety and Health, Morgantown, West Virginia3

Received 30 March 2005/ Returned for modification 15 June 2005/ Accepted 22 June 2005

A double-immunostaining halogen immunoassay was developed to identify aerosolized conidia, hyphae, and fragments of Alternaria alternata by using an anti-Alternaria polyclonal antiserum, while, simultaneously, allergy to these components was concurrently determined by using human immunoglobulin E antibodies.

* Corresponding author. Mailing address: Woolcock Allergen Unit, Room 461, Blackburn Building, D06, Department of Medicine, The University of Sydney, NSW, Australia 2006. Phone: 61 2 9351 2093. Fax: 61 2 9351 7451. E-mail: ert{at}mail.med.usyd.edu.au.

Clinical and Diagnostic Laboratory Immunology, September 2005, p. 1114-1116, Vol. 12, No. 9
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.9.1114-1116.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»