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 Previous Article

Clinical and Diagnostic Laboratory Immunology, July 2005, p. 885-887, Vol. 12, No. 7
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.7.885-887.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Development of Rapid Immunochromatographic Test with Recombinant NcSAG1 for Detection of Antibodies to Neospora caninum in Cattle

Min Liao,1 Shoufa Zhang,2 Xuenan Xuan,1* Guohong Zhang,1 Xiaohong Huang,1 Ikuo Igarashi,1 and Kozo Fujisaki1

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan,1 Department of Veterinary Medicine, Yanbian University, Longjing, Jilin Province 133400, China2

Received 26 December 2004/ Returned for modification 9 February 2005/ Accepted 19 April 2005

An immunochromatographic test (ICT) with recombinant surface antigen 1 of Neospora caninum (NcSAG1) was developed for the rapid detection of antibodies to N. caninum in cattle. The ICT was used to clearly discriminate between immunofluorescent-antibody test (IFAT)-positive bovine sera and IFAT-negative bovine sera. Serum samples collected from cattle in Yanbian, China, were examined by the ICT. Of the 96 serum samples, 23 (24.0%) were positive by the ICT, and 19 (19.8%) samples were positive by a previously developed enzyme-linked immunosorbent assay (ELISA). Eighteen of 19 ELISA-positive samples were positive according to the ICT. A good agreement was found between the results of the ICT and the ELISA. The results presented here suggest that the ICT with recombinant truncated NcSAG1 fused to glutathione S-transferase is a useful and reliable method for the detection of antibodies to N. caninum in cattle.

* Corresponding author. Mailing address: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan. Phone: 81-155-49-5648. Fax: 81-155-49-5643. E-mail: gen{at}obihiro.ac.jp.

Clinical and Diagnostic Laboratory Immunology, July 2005, p. 885-887, Vol. 12, No. 7
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.7.885-887.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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