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Clinical and Diagnostic Laboratory Immunology, July 2005, p. 821-824, Vol. 12, No. 7
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.7.821-824.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Multiplexed Particle-Based Anti-Granulocyte Macrophage Colony Stimulating Factor Assay Used as Pulmonary Diagnostic Test

Tracey L. Bonfield,1* Nejimol John,1 Barbara P. Barna,1 Mani S. Kavuru,1 Mary Jane Thomassen,1,3 and Belinda Yen-Lieberman2

Departments of Pulmonary, Allergy and Critical Care Medicine,1 Clinical Pathology,2 Cell Biology, The Cleveland Clinic Foundation, Cleveland, Ohio 44195-50383

Received 18 January 2005/ Returned for modification 2 March 2005/ Accepted 18 April 2005

Pulmonary alveolar proteinosis (PAP) is characterized by the accumulation of lipoproteinaceous material within the lung alveoli. Recent studies indicate that PAP is an autoimmune disease characterized by a neutralizing anti-granulocyte macrophage colony stimulating factor (GM-CSF) antibody. At present the only definitive diagnostic test for PAP is open lung biopsy. We have previously published that anti-GM-CSF is diagnostic for PAP and correlates with disease pathogenesis using a traditional serial anti-GM-CSF antibody titer format (T. L. Bonfield, M. S. Kavuru, and M. J. Thomassen, Clin. Immunol. 105:342-350, 2002). Titer analysis is a semiquantitative method, and often subtle changes in antibody titer are not detectable. In this report we present data to support anti-GM-CSF detection by a quantitative highly sensitive multiplexed particle-based assay which has the potential to be a clinical diagnostic test.

* Corresponding author. Mailing address: Department of Pulmonary, Allergy and Critical Care Medicine, 9500 Euclid Avenue, Cleveland Clinic Foundation, Desk A90, Cleveland, Ohio 44195-5038. Phone: (216) 445-7298. Fax: (216) 444-5172. E-mail: bonfiet{at}ccf.org.

Clinical and Diagnostic Laboratory Immunology, July 2005, p. 821-824, Vol. 12, No. 7
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.7.821-824.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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