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 Previous Article

Clinical and Diagnostic Laboratory Immunology, May 2005, p. 683-684, Vol. 12, No. 5
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.5.683-684.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Construction and Expression of Recombinant Streptolysin-O and Preevaluation of Its Use in Immunoassays

Blanca Velázquez,1 Hugo Massaldi,1 Julio Battistoni,2 and José A. Chabalgoity1*

Department of Biotechnology, Institute of Hygiene, School of Medicine, Universidad de la República, Uruguay, Av. Alfredo Navarro 3051, Montevideo, Uruguay,1 Laboratory of Immunotechnology, Department of Immunology, School of Chemistry-School of Science, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay2

Received 22 November 2004/ Returned for modification 3 January 2005/ Accepted 14 March 2005

Commercially available immunoassays for assessment of anti-streptolysin-O antibodies use native streptolysin-O obtained by a complex process. We prepared a biologically active recombinant streptolysin-O with higher yield and a simpler purification process. An enzyme-linked immunosorbent assay developed with this recombinant showed good correlation with a commercial test, suggesting that it could be suitable for immunoassays.

* Corresponding author. Mailing address: Laboratory for Vaccine Research, Department of Biotechnology, Institute of Hygiene, School of Medicine, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, R.O. Uruguay. Phone: (00598) 2 4871288, ext. 1120. Fax: (00598) 2 4871288, ext. 1102. E-mail: jachabal{at}higiene.edu.uy.

Clinical and Diagnostic Laboratory Immunology, May 2005, p. 683-684, Vol. 12, No. 5
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.5.683-684.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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