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Rapid Immunofluorescence Microscopy for Diagnosis of Melioidosis

Faculty of Tropical Medicine, Wellcome Trust-Oxford University-Mahidol University Tropical Medicine Research Program,¹ Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand,² Menzies School of Health Research, Charles Darwin University, and Northern Territory Clinical School, Flinders University, Darwin, Australia,³ Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom⁴

Received 26 November 2004/ Returned for modification 4 January 2005/ Accepted 14 January 2005

An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were prospectively compared with the culture results for 776 specimens from patients with suspected melioidosis. The sensitivities of both IF tests were 66%, and the specificities were 99.5 and 99.4%, respectively.

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