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Clinical and Diagnostic Laboratory Immunology, March 2005, p. 465-473, Vol. 12, No. 3
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.3.465-473.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Glycolipids of Mycobacterium tuberculosis Strain H37Rv Are Potential Serological Markers for Diagnosis of Active Tuberculosis

R. P. Tiwari,1 Dileep Tiwari,1 Sanjay K. Garg,1 Ramesh Chandra,1 and Prakash S. Bisen1*

Department of Biotechnology, J. C. Bose Institute of Life Sciences, Bundelkhand University, Jhansi, India1

Received 16 August 2004/ Returned for modification 14 October 2004/ Accepted 12 November 2004

A simple and cost-effective diagnostic tool (TB Screen Test) for the screening of patients with pulmonary and extrapulmonary tuberculosis and for differentiation of those individuals from individuals without tuberculosis, other common infections, and healthy controls has been developed. The serological responses of purified mycobacterial glycolipid antigens were examined by a liposome agglutination assay. The assay was able to detect very low antiglycolipid antibody concentrations in the infected individuals. The sera from the tuberculosis patient group had significantly higher concentrations of antiglycolipid antibody than the sera from uninfected control subjects, with 94% sensitivity and 98.3% specificity. Glycolipids of Mycobacterium tuberculosis H37Rv antigens were isolated, purified, and characterized. After interchelation with liposome particles, these purified antigens specifically bound to the antiglycolipid antibodies present in the sera of patients with tuberculosis, resulting in the formation of a blue agglutination. This protocol clearly differentiates healthy controls and M. bovis BCG-vaccinated subjects from those with active tuberculosis. The resultant diagnostic tool, the TB Screen Test, is more economical and rapid (4 min) than other currently available products and can be used for the mass screening of a heavily afflicted population.

* Corresponding author. Mailing address: Department of Biotechnology, J. C. Bose Institute of Life Sciences, Bundelkhand University, Jhansi 284218 U.P., India. Phone: 91-517-2320497. Fax: 91-517-2320761. E-mail: prakash_bisen{at}hotmail.com.

Clinical and Diagnostic Laboratory Immunology, March 2005, p. 465-473, Vol. 12, No. 3
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.3.465-473.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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