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Clinical and Diagnostic Laboratory Immunology, October 2005, p. 1141-1144, Vol. 12, No. 10
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.10.1141-1144.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Sensitivity and Specificity of a Monoclonal Antibody-Based Fluorescence Assay for Detecting Enterocytozoon bieneusi Spores in Feces of Simian Immunodeficiency Virus-Infected Macaques

Inderpal Singh,1 Abhineet S. Sheoran,1 Quanshun Zhang,1 Angela Carville,2 and Saul Tzipori1*

Division of Infectious Diseases, Tufts University Cummings School of Veterinary Medicine, North Grafton, Massachusetts,1 Harvard Medical School New England Regional Primate Research Center, Southborough, Massachusetts2

Received 6 June 2005/ Returned for modification 18 July 2005/ Accepted 26 July 2005

Enterocytozoon bieneusi is clinically the most significant among the microsporidia causing chronic diarrhea, wasting, and cholangitis in individuals with human immunodeficiency virus/AIDS. Microscopy with either calcofluor or modified trichrome stains is the standard diagnostic test for microsporidiosis and does not allow species identification. Detection of E. bieneusi infection based on PCR is limited to a few reference laboratories, and thus it is not the standard diagnostic assay. We have recently reported the development and characterization of a panel of monoclonal antibodies against E. bieneusi, and in this publication we evaluated the specificity and sensitivity of an immunofluorescence assay (IFA), compared with PCR, in simian immunodeficiency virus-infected macaques. The IFA, which correlated with the primary PCR method, with a detection limit of 1.5 x105 spores per gram of feces, will simplify considerably the detection of E. bieneusi spores in clinical and environmental specimens and in laboratory and epidemiological investigations.

* Corresponding author. Mailing address: Division of Infectious Diseases, Tufts University Cummings School of Veterinary Medicine, 200 Westborough Road, North Grafton, MA 01536. Phone: (508) 839-7955. Fax: (508) 839-7911. E-mail: saul.tzipori{at}tufts.edu.

Clinical and Diagnostic Laboratory Immunology, October 2005, p. 1141-1144, Vol. 12, No. 10
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.10.1141-1144.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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