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Clinical and Diagnostic Laboratory Immunology, January 2005, p. 40-43, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.40-43.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Prevalence of Antibodies to Onchocerca volvulus in Residents of Oaxaca, Mexico, Treated for 10 Years with Ivermectin

Alberto Gómez-Priego,1,2 Raymundo Mendoza,1 and Jorge-Luis de-la-Rosa1*

Laboratorio de Helmintos Tisulares, Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaría de Salud, Santo Tomás, Miguel Hidalgo,1 Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, México2

Received 30 January 2004/ Returned for modification 8 September 2004/ Accepted 22 October 2004

Studies to determine the prevalence of antibodies to Onchocerca volvulus, prior to and after actions carried out to interrupt transmission, are scarce in Mexico. Here we report the prevalence of immunoglobulin G (IgG) and IgG4 antibodies in an enzyme-linked immunosorbent assay (ELISA) against a crude extract of O. volvulus adult worm in serum samples from persons under noninterrupted biannual treatment with ivermectin in areas of onchocercosis endemicity in Mexico. To perform the prevalence studies, the ELISA procedures were first evaluated. Serological studies were performed with serum samples from skin microfilaria carriers from Guatemala and from people microfilariodermic negative living in the same area as the Guatemalan patients. Sensitivity values for IgG or IgG4 detection were 71 and 86%, while specificities were 92 and 100%, respectively. No anti-O. volvulus antibodies were found in samples from nonendemic controls from Mexico, but 3 of 71 samples from residents in the onchocercosis area of Oaxaca, Mexico, and who have been under ivermectin treatment during the last 10 years were only positive to IgG. Notwithstanding that the IgG4 isotype was not detected and a low (4.2%) anti-O. volvulus IgG antibody prevalence was found, a seroepidemiological follow-up must be performed in order to confirm interruption of onchocercosis transmission in the area of Oaxaca, Mexico, in which onchocercosis is endemic.

* Corresponding author. Mailing address: Laboratorio de Helmintos Tisulares, Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaría de Salud, Carpio 470, Santo Tomás, Miguel Hidalgo, México D.F. 11340, México. Phone: 52-55-53427550, ext. 224. Fax: 525-55-53413264. E-mail address for J.-L. de-la-Rosa: jldelarosa{at}salud.gob.mx. E-mail address for A. G. Priego: E-mail: agpriego{at}prodigy.net.mx.

Clinical and Diagnostic Laboratory Immunology, January 2005, p. 40-43, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.40-43.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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