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Clinical and Diagnostic Laboratory Immunology, January 2005, p. 228-230, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.228-230.2005

Effects of Lymphocyte Isolation and Timing of Processing on Detection of CD127 Expression on T Cells in Human Immunodeficiency Virus-Infected Patients

Jeanette Higgins,1 Julia A. Metcalf,2 Randy A. Stevens,1 Michael Baseler,1 Martha C. Nason,2 H. Clifford Lane,2 and Irini Sereti2*

AIDS Monitoring Laboratory, Clinical Services Program, Science Applications International Corp., Frederick,1 National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland2

Received 19 August 2004/ Returned for modification 14 September 2004/ Accepted 25 October 2004

Decreases in the detection of CD127 expression on T cells of human immunodeficiency virus-infected patients by flow cytometry can occur by delayed processing or by peripheral blood mononuclear cell isolation and cryopreservation. These observations should be considered in the interpretation of functional studies and the planning of multicenter clinical trials.

* Corresponding author. Mailing address: Clinical and Molecular Retrovirology Section, Laboratory of Immunoregulation, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bldg 10, Room 11B-04, 10 Center Dr., MSC 1876, Bethesda, MD 20892. Phone: (301) 496-5533. Fax: (301) 402-0070. E-mail: isereti{at}niaid.nih.gov.

Clinical and Diagnostic Laboratory Immunology, January 2005, p. 228-230, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.228-230.2005






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