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Clinical and Diagnostic Laboratory Immunology, January 2005, p. 224-227, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.224-227.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison of a New, Affordable Flow Cytometric Method and the Manual Magnetic Bead Technique for CD4 T-Lymphocyte Counting in a Northern Nigerian Setting

Godwin E. Imade,1* Bitrus Badung,1 Sunday Pam,1 Oche Agbaji,1 Daniel Egah,1 Atiene S. Sagay,1 Jean-Louis Sankalé,2 Saidi Kapiga,2 John Idoko,1 and Phyllis Kanki2

AIDS Prevention Initiative Nigeria, Jos University Teaching Hospital, Jos, Nigeria,1 Harvard School of Public Health, Boston, Massachusetts2

Received 12 August 2004/ Returned for modification 13 October 2004/ Accepted 3 November 2004

We compared two techniques for CD4 T-lymphocyte counting: flow cytometry (Cyflow) and magnetic beads (Dynabead). Similar results with good correlation were obtained from the 40 adult blood samples counted (P = 0.057, r = 0.93). The Cyflow technique is more precise and cost-effective than the Dynabead method ($3 to $5 versus $12 to $22 per test, respectively), since as many as 200 samples can be measured per day.

* Corresponding author. Mailing address: Department of Obstetrics and Gynaecology, Faculty of Medical Sciences, University of Jos, P.M.B. 2084, Jos, Nigeria. Phone: 234 8035860811. Fax: 234 73 456469. E-mail: ereimade{at}yahoo.co.uk.

Clinical and Diagnostic Laboratory Immunology, January 2005, p. 224-227, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.224-227.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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