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Clinical and Diagnostic Laboratory Immunology, January 2005, p. 206-212, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.206-212.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Differentiation, Maturation, and Survival of Dendritic Cells by Osteopontin Regulation

Kodai Kawamura, Kazuhiro Iyonaga,* Hidenori Ichiyasu, Junji Nagano, Moritaka Suga, and Yutaka Sasaki

Department of Respiratory Medicine, Graduate School of Medical Sciences, Kumamoto University, Honjo, Kumamoto, Japan

Received 1 July 2004/ Returned for modification 8 September 2004/ Accepted 27 October 2004

Dendritic cells (DCs) are antigen-presenting cells with the ability to induce primary immune responses necessary in innate immunity and adaptive immunity. Osteopontin (OPN) is a secreted acidic phosphoprotein containing an arginine-glycine-aspartate sequence and has been suggested to play an important role in early cellular immune responses. The interaction between DCs and OPN has not been clarified. We hypothesized that there is an important interaction between DCs and OPN, which is an indispensable extracellular matrix component in early cellular immune responses. Human monocyte-derived DCs synthesized OPN especially during the differentiation from monocytes to immature DCs. By blocking of OPN with anti-OPN antibody, cultured DCs became smaller and expressed lower levels of costimulatory molecules and major histocompatibility complex class II antigens than untreated DCs. Furthermore, DCs treated with anti-OPN antibody easily underwent apoptosis. These results suggest that human DCs can produce OPN and that OPN may play a role in the differentiation, maturation, and survival of DCs by autocrine and/or paracrine pathways.

* Corresponding author. Mailing address: Department of Respiratory Medicine, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan. Phone: 81-96-373-5012. Fax: 81-96-373-5012. E-mail: iyonaga{at}kaiju.medic.kumamoto-u.ac.jp.

Clinical and Diagnostic Laboratory Immunology, January 2005, p. 206-212, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.206-212.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Renkl, A. C., Wussler, J., Ahrens, T., Thoma, K., Kon, S., Uede, T., Martin, S. F., Simon, J. C., Weiss, J. M. (2005). Osteopontin functionally activates dendritic cells and induces their differentiation toward a Th1-polarizing phenotype. Blood 106: 946-955 [Abstract] [Full Text]  


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