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Clinical and Diagnostic Laboratory Immunology, January 2005, p. 157-164, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.157-164.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Induction of Thymus-Derived {gamma}{delta} T Cells by Escherichia coli Enterotoxin B Subunit in Peritoneal Cavities of Mice

Toshiyasu Shimizu,1 Keiko Sasaki,1 Michio Kato,1 Hideyuki Arimitsu,1 Sadayuki Ochi,1 Naoko Shigemori,1 Eddy Bagus Wasito,2 Takashi Yokochi,3 and Takao Tsuji1*

Department of Microbiology, Fujita Health University School of Medicine, Tokyoake,1 Department of Microbiology, Aichi Medical University, Nagakute, Aichi, Japan,3 Department of Microbiology, Tropical Disease Center, Airlangga University, Unair, JL, Mulyorejo, Surabaya, Indonesia2

Received 20 July 2004/ Returned for modification 10 September 2004/ Accepted 23 September 2004

We examined the activation of intraperitoneal T cells in BALB/c mice by the Escherichia coli enterotoxin B subunit, which induced a specific Th2 type of T-cell response to intraperitoneally coadministered bovine immunoglobulin G. The numbers of both {gamma}{delta} and {alpha}ß T cells increased significantly after intraperitoneal administration of the B subunit in a time-dependent manner; these numbers were not affected by the B-subunit G33D mutant, which is defective in GM1 ganglioside-binding ability. Early after administration a small number of {gamma}{delta} T cells produced either interleukin-4 (IL-4) or gamma interferon, while late after administration primarily IL-10-producing {gamma}{delta} T cells were detected. {gamma}{delta} T cells induced by the B subunit did not express a characteristic V gene over the time course of the study. The induction of {gamma}{delta} T cells did not occur in athymic nu/nu mice but could be induced upon transplantation of fetal AKR thymus-like {alpha}ß T cells. {gamma}{delta} T cells in athymic nu/nu mice with a fetal thymic graft predominantly expressed the donor Thy-1.1 antigen but not the host Thy-1.2 antigen. The induction of these T cells, however, could not be restored by coadministration of the B subunit with peritoneal cells from normal mice. These results suggest that the B subunit activates intraperitoneal {gamma}{delta} and {alpha}ß T cells in a manner dependent upon its ability to bind to GM1 ganglioside. {gamma}{delta} T cells induced by the B subunit are Th2-type cells derived from the thymus. These {gamma}{delta} T cells may be functionally involved in specific Th2 responses to the B subunit, which possibly acts as an adjuvant through the influence of {alpha}ß T cells.

* Corresponding author. Mailing address: Department of Microbiology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan. Phone: 81-562-93-2432. Fax: 81-562-93-4003. E-mail: ttsuji{at}fujita-hu.ac.jp.

Clinical and Diagnostic Laboratory Immunology, January 2005, p. 157-164, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.157-164.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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