This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Maeda, K.
Right arrow Articles by Matsumura, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Maeda, K.
Right arrow Articles by Matsumura, T.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, January 2005, p. 122-124, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.122-124.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of Another B-Cell Epitope in the Type-Specific Region of Equine Herpesvirus 4 Glycoprotein G

Ken Maeda,1* Fuminori Mizukoshi,1 Masataka Hamano,1 Kazushige Kai,1 Takashi Kondo,2 and Tomio Matsumura2

Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Yoshida, Yamaguchi,1 Epizootic Research Station, Equine Research Institute, Japan Racing Association, Shiba, Kokubunji-Machi, Shimotsuga-gun, Tochigi, Japan2

Received 19 July 2004/ Returned for modification 28 September 2004/ Accepted 26 October 2004

Recently, a novel 12-mer B-cell epitope, MKNNPIYSEGSL, in the type-specific region of equine herpesvirus 1 (EHV-1) glycoprotein G (gG) was identified and used as an antigen for enzyme-linked immunosorbent assay (Maeda et al., J. Clin. Microbiol. 42:1095-1098, 2004). Although our prototype strain, TH20p, possesses two repeat sequences containing the B-cell epitope, the EHV-4 NS80567 strain has two repeat sequences that are not identical. One repeat sequence stretch contained the B-cell epitope, while the other contained the 11-mer, MKNNPVYSESL (underlining indicates a different amino acid). In this study, heterogeneity of the type-specific region was compared among Japanese EHV-4 isolates. The 11-mer peptide, MKNNPVYSESL, specifically reacted with sera from horses naturally infected with EHV-4 but not with sera from horses experimentally infected with EHV-4 TH20p. The 11-mer peptide may be another B-cell epitope in the type-specific region.

* Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan. Phone and fax: 81-83-933-5887. E-mail: kmaeda{at}yamaguchi-u.ac.jp.

Clinical and Diagnostic Laboratory Immunology, January 2005, p. 122-124, Vol. 12, No. 1
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.1.122-124.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»