This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McLaren, P. J.
Right arrow Articles by Fowke, K. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McLaren, P. J.
Right arrow Articles by Fowke, K. R.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, September 2004, p. 977-982, Vol. 11, No. 5
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.5.977-982.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Antigen-Specific Gene Expression Profiles of Peripheral Blood Mononuclear Cells Do Not Reflect Those of T-Lymphocyte Subsets

Paul J. McLaren,1 Michael Mayne,2 Stuart Rosser,1,3 Teri Moffatt,2 Kevin G. Becker,4 Francis A. Plummer,1 and Keith R. Fowke1*

Department of Medical Microbiology,1 Department of Pharmacology and Therapeutics,2 Department of Internal Medicine, Section of Infectious Diseases, University of Manitoba, Winnipeg, Canada,3 DNA Array Unit, National Institute on Aging, Bethesda, Maryland4

Received 23 April 2004/ Returned for modification 28 June 2004/ Accepted 1 July 2004

Advances in microarray technology have allowed for the monitoring of thousands of genes simultaneously. This technology is of particular interest to immunologists studying infectious diseases, because it provides tremendous potential for investigating host-pathogen interactions at the level of immune gene expression. To date, many studies have focused either on cell lines, where the physiological relevance is questionable, or on mixed cell populations, where the contributions of individual subpopulations are unknown. In the present study, we perform an intrasubject comparison of antigen-stimulated immune gene expression profiles between a mixed population of peripheral blood mononuclear cells (PBMC) and the two predominant cell types found in PBMC, CD4+ and CD8+ T lymphocytes. We show that the microarray profiles of CD4+ and CD8+ T lymphocytes differ from each other as well as from that of the mixed cell population. The independence of the gene expression profiles of different cell types is demonstrated with a ubiquitous antigen (Candida albicans) as well as with a disease-specific antigen (human immunodeficiency virus p24). This study has important implications for microarray studies of host immunity and underscores the importance of profiling the expression of specific cell types.

* Corresponding author. Mailing address: Department of Medical Microbiology, University of Manitoba, 539-730 William Ave., Winnipeg, Manitoba, Canada R3E 0W3. Phone: (204) 789-3818. Fax: (204) 789-3926. E-mail: fowkekr{at}cc.umanitoba.ca.

Clinical and Diagnostic Laboratory Immunology, September 2004, p. 977-982, Vol. 11, No. 5
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.5.977-982.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»