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Clinical and Diagnostic Laboratory Immunology, September 2004, p. 849-855, Vol. 11, No. 5
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.5.849-855.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Antigen Recognition by Serum Antibodies in White-Tailed Deer (Odocoileus virginianus) Experimentally Infected with Mycobacterium bovis

W. R. Waters,1* M. V. Palmer,1 J. P. Bannantine,1 D. L. Whipple,1 R. Greenwald,2 J. Esfandiari,2 P. Andersen,3 J. McNair,4 J. M. Pollock,4 and K. P. Lyashchenko2

National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Bacterial Diseases of Livestock Research Unit, Ames, Iowa,1 Chembio Diagnostic Systems, Inc., Medford, New York,2 Bacteriology Department, Veterinary Sciences Division, Stormont, Belfast, United Kingdom,4 Statens Serum Institut, Copenhagen, Denmark3

Received 2 April 2004/ Accepted 13 July 2004

White-tailed deer (Odocoileus virginianus) have emerged as reservoirs of bovine tuberculosis in northern America. For tuberculosis surveillance of deer, antibody-based assays are particularly attractive because deer are handled only once and immediate processing of the sample is not required. Sera collected sequentially from 25 Mycobacterium bovis-infected and 7 noninfected deer were evaluated by enzyme-linked immunosorbent assay (ELISA), immunoblotting, and multiantigen print immunoassay (MAPIA) for immunoglobulin specific to M. bovis antigens. Various routes of experimental M. bovis infection, such as intratonsillar inoculation (n = 11), aerosol (n = 6), and exposure to infected deer (in contact, n = 8), were studied. Upon infection, specific bands of reactivity at ~24 to 26 kDa, ~33 kDa, ~42 kDa, and ~75 kDa to M. bovis whole-cell sonicate were detected by immunoblot. Lipoarabinomannan-specific immunoglobulin was detected as early as 36 days postchallenge, and responses were detected for 94% of intratonsillarly and "in-contact"-infected deer. In MAPIA, sera were tested with 12 native and recombinant antigens coated on nitrocellulose. All in-contact-infected (8 of 8) and 10 of 11 intratonsillarly infected deer produced antibody reactive with one or more of the recombinant/native antigens. Responses were boosted by injection of tuberculin for intradermal tuberculin skin testing. Additionally, three of six deer receiving a very low dose of M. bovis via aerosol exposure produced antibody specific to one or more recombinant proteins. M. bovis was isolated from one of three nonresponding aerosol-challenged deer. Of the 12 antigens tested, the most immunodominant protein was MPB83; however, a highly sensitive serodiagnostic test will likely require use of multiple antigens.

* Corresponding author. Mailing address: National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, P.O. Box 70, Ames, IA 50010-0070. Phone: (515) 663-7756. Fax: (515) 663-7458. E-mail: rwaters{at}nadc.ars.usda.gov.

Clinical and Diagnostic Laboratory Immunology, September 2004, p. 849-855, Vol. 11, No. 5
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.5.849-855.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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  • O'Brien, D. J., Schmitt, S. M., Lyashchenko, K. P., Waters, W. R., Berry, D. E., Palmer, M. V., McNair, J., Greenwald, R., Esfandiari, J., Cosgrove, M. K. (2009). EVALUATION OF BLOOD ASSAYS FOR DETECTION OF MYCOBACTERIUM BOVIS IN WHITE-TAILED DEER (ODOCOILEUS VIRGINIANUS) IN MICHIGAN. J Wildl Dis 45: 153-164 [Abstract] [Full Text]  
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