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Clinical and Diagnostic Laboratory Immunology, September 2004, p. 825-834, Vol. 11, No. 5
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.5.825-834.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Serological Response to Pasteurella multocida NanH Sialidase in Persistently Colonized Rabbits

Susan Sanchez,1 Shaikh Mizan,2 Charlotte Quist,1 Patricia Schroder,2 Michelle Juneau,1 Donald Dawe,2 Branson Ritchie,3 and Margie D. Lee2*

Athens Diagnostic Laboratory,1 Department of Infectious Diseases,2 Department of Small Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia3

Received 1 March 2004/ Returned for modification 5 May 2004/ Accepted 21 May 2004

Pasteurella multocida is a mucosal pathogen that colonizes the upper respiratory system of rabbits. Respiratory infections can result, but the bacteria can also invade the circulatory system, producing abscesses or septicemia. P. multocida produces extracellular sialidase activity, which is believed to augment colonization of the respiratory tract and the production of lesions in an active infection. Previously, it was demonstrated that some isolates of P. multocida contain two unique sialidase genes, nanH and nanB, that encode enzymes with different substrate specificities (S. Mizan, A. D. Henk, A. Stallings, M. Meier, J. J. Maurer, and M. D. Lee, J. Bacteriol. 182:6874-6883, 2000). We developed a recombinant antigen enzyme-linked immunosorbent assay (ELISA) based on the NanH sialidase of P. multocida and demonstrated that rabbits that were experimentally colonized with P. multocida produce detectable anti-NanH immunoglobulin M (IgM) and IgG in serum, although they demonstrated no clinical signs of pasteurellosis. In addition, clinically ill pet rabbits infected with P. multocida possessed IgM and/or IgG antibody against NanH. The NanH ELISA may be useful for the diagnosis of P. multocida infections in sick rabbits as well as for screening for carriers in research rabbit colonies.

* Corresponding author. Mailing address: Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA 30602. Phone: (706) 583-0797. Fax: (706) 542-5630. E-mail: leem{at}vet.uga.edu.

Clinical and Diagnostic Laboratory Immunology, September 2004, p. 825-834, Vol. 11, No. 5
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.5.825-834.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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