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Clinical and Diagnostic Laboratory Immunology, March 2004, p. 379-386, Vol. 11, No. 2
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.2.379-386.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Establishment of an Animal Model Using Recombinant NOD.B10.D2 Mice To Study Initial Adhesion of Oral Streptococci

Mohammad Abdus Salam,1,2 Naoko Matsumoto,1 Khairul Matin,2 Yuzo Tsuha,1 Ryoma Nakao,1 Nobuhiro Hanada,2 and Hidenobu Senpuku1*

Department of Bacteriology, National Institute of Infectious Diseases,1 Department of Oral Health, National Institute of Public Health, Shinjuku-ku, Tokyo 162-8640, Japan2

Received 4 September 2003/ Returned for modification 19 October 2003/ Accepted 28 December 2003

An oral biofilm is a community of surface-attached microorganisms that coats the oral cavity, including the teeth, and provides a protective reservoir for oral microbial pathogens, which are the primary cause of persistent and chronic infectious diseases in patients with dry mouth or Sjögren's syndrome (SS). The purpose of this study was to establish an animal model for studying the initial adhesion of oral streptococci that cause biofilm formation in patients with dry mouth and SS in an attempt to decrease the influence of cariogenic organisms and their substrates. In nonobese diabetogenic (NOD) mice that spontaneously develop insulin-dependent diabetes mellitus (IDDM) and SS, we replaced major histocompatibility complex (MHC) class II (Ag7 Eg7) and class I Db with MHC class II (Ad Ed) and class I Dd from nondiabetic B10.D2 mice to produce an animal model that inhibited IDDM without affecting SS. The adhesion of oral streptococci, including Streptococcus mutans, onto tooth surfaces was then investigated and quantified in homologous recombinant N5 (NOD.B10.D2) and N9 (NOD.B10.D2) mice. We found that a higher number of oral streptococci adhered to the tooth surfaces of N5 (NOD.B10.D2) and N9 (NOD.B10.D2) mice than to those of the control C57BL/6 and B10.D2 mice. On the basis of our observation, we concluded that these mouse models might be useful as animal models of dry mouth and SS for in vivo biological studies of oral biofilm formation on the tooth surfaces.

* Corresponding author. Mailing address: Department of Bacteriology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan. Phone: 81-3-5285-1111, ext. 2222. Fax: 81-3-5285-1172. E-mail: hsenpuku{at}nih.go.jp.

Clinical and Diagnostic Laboratory Immunology, March 2004, p. 379-386, Vol. 11, No. 2
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.2.379-386.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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