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Clinical and Diagnostic Laboratory Immunology, March 2004, p. 235-238, Vol. 11, No. 2
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.2.235-238.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Antibody Response of Healthy Adults to Recombinant Thrombospondin-Related Adhesive Protein of Cryptosporidium 1 after Experimental Exposure to Cryptosporidium Oocysts

The University of Texas Houston Medical School and School of Public Health, Houston, Texas,¹ Laboratory of Parasitology, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts,³ Imperial College, Department of Biology, London, United Kingdom,² Department of Infectious Parasitic and Immunomediated Diseases, Instituto Superiore di Sanita, Rome, Italy⁴

Received 20 October 2003/ Returned for modification 13 November 2003/ Accepted 20 November 2003

Thrombospondin-related adhesive protein of Cryptosporidium 1 (TRAP-C1) belongs to a group of proteins that are also found in Toxoplasma gondii, Eimeria tenella, and Plasmodium species. TRAP-related proteins are needed for gliding motility, host-cell attachment, and invasion. The objective of this study was to characterize the antibody response to recombinant TRAP-C1 (rTRAP-C1) in healthy volunteers exposed to C. parvum and their association with clinical illness. A total of 31 healthy adult volunteers participated. Seven volunteers received the C. parvum TAMU isolate (inocula, 10 to 300 oocysts), and 24 volunteers received the C. parvum UCP isolate (500 to 10⁵ oocysts). The total antibody (immunoglobulin M [IgM], IgG, and IgA) response to rTRAP C-1 was measured by enzyme-linked immunosorbent assays prior to and after exposure to Cryptosporidium parvum (days 0 to 45). Results of this study showed that individuals who were uninfected demonstrated higher reactivity at baseline compared to those who became infected. After challenge, increases in antibody reactivity were seen on days 30 and 45 compared to the results seen on days 0 to 5. The increases in antibody reactivity were statistically significant in subjects with diarrhea and with or without detectable oocysts compared to the results seen with those who were uninfected and asymptomatic. These findings suggest that increases in antibody reactivity to rTRAP-C1 occur after recent exposure to C. parvum.

This article has been cited by other articles:

• Kjos, S. A., Jenkins, M., Okhuysen, P. C., Chappell, C. L. (2005). Evaluation of Recombinant Oocyst Protein CP41 for Detection of Cryptosporidium-Specific Antibodies. CVI 12: 268-272 [Abstract] [Full Text]