This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Christopherson, J. A.
Right arrow Articles by Schell, R. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Christopherson, J. A.
Right arrow Articles by Schell, R. F.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, January 2003, p. 44-52, Vol. 10, No. 1
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.1.44-52.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Destructive Arthritis in Vaccinated Interferon Gamma-Deficient Mice Challenged with Borrelia burgdorferi: Modulation by Tumor Necrosis Factor Alpha

John A. Christopherson,1,2 Erik L. Munson,1,2,{dagger} Douglas M. England,3,4 Cindy L. Croke,1,2 Monica C. Remington,1,2 Melanie L. Molitor,1,2 David J. DeCoster,1,2 Steven M. Callister,5,6 and Ronald F. Schell1,2,7*

Wisconsin State Laboratory of Hygiene,1 Departments of Medical Microbiology and Immunology,2 Pathology and Laboratory Medicine,3 Bacteriology, University of Wisconsin, Madison, Wisconsin 53706,7 Department of Pathology, Meriter Hospital, Madison, Wisconsin 53715,4 Microbiology Research Laboratory,5 Department of Infectious Diseases, Gundersen Lutheran Medical Center, La Crosse, Wisconsin 546016

Received 19 June 2002/ Returned for modification 29 August 2002/ Accepted 26 September 2002

We found that Borrelia burgdorferi-vaccinated gamma interferon-deficient (IFN-{gamma}0) mice challenged with B. burgdorferi developed prominent chronic destructive osteoarthropathy. When these mice were treated with anti-tumor necrosis factor alpha (TNF-{alpha}) antibody, the severity of the destructive osteoarthritis was enhanced and affected the mobility of the animals. In addition, extensive swelling of the hind paws occurred. In contrast, treatment of B. burgdorferi-vaccinated, challenged IFN-{gamma}0 mice with recombinant TNF-{alpha} (rTNF-{alpha}) inhibited the development of arthritis, including swelling of the hind paws. Moreover, treatment of vaccinated, challenged IFN-{gamma}0 mice with anti-TNF-{alpha} inhibited fourfold the production of an antibody that kills B. burgdorferi, while treatment of vaccinated, challenged IFN-{gamma}0 mice with rTNF-{alpha} slightly elevated the level of the borreliacidal antibody. These results suggest that the level of TNF-{alpha} directly or indirectly regulates the production of borreliacidal antibody and the development of vaccine-induced destructive Lyme osteoarthritis. Studies are in progress to determine the mechanism by which TNF-{alpha}-dependent cytokines generate the destructive arthritis.

* Corresponding author. Mailing address: University of Wisconsin, Wisconsin State Laboratory of Hygiene, 465 Henry Mall, Madison, WI 53706. Phone: (608) 262-3634. Fax: (608) 265-3451. E-mail: RFschell{at}facstaff.wisc.edu.

{dagger} Present address: Medical Science Laboratories, Wauwatosa, WI 53226.

Clinical and Diagnostic Laboratory Immunology, January 2003, p. 44-52, Vol. 10, No. 1
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.1.44-52.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nardelli, D. T., Callister, S. M., Schell, R. F. (2008). Lyme Arthritis: Current Concepts and a Change in Paradigm. CVI 15: 21-34 [Full Text]  
  • Nardelli, D. T., Cloute, J. P., Luk, K. H. K., Torrealba, J., Warner, T. F., Callister, S. M., Schell, R. F. (2005). CD4+ CD25+ T Cells Prevent Arthritis Associated with Borrelia Vaccination and Infection. CVI 12: 786-792 [Abstract] [Full Text]  
  • Nardelli, D. T., Burchill, M. A., England, D. M., Torrealba, J., Callister, S. M., Schell, R. F. (2004). Association of CD4+ CD25+ T Cells with Prevention of Severe Destructive Arthritis in Borrelia burgdorferi-Vaccinated and Challenged Gamma Interferon-Deficient Mice Treated with Anti-Interleukin-17 Antibody. CVI 11: 1075-1084 [Abstract] [Full Text]  
  • Burchill, M. A., Nardelli, D. T., England, D. M., DeCoster, D. J., Christopherson, J. A., Callister, S. M., Schell, R. F. (2003). Inhibition of Interleukin-17 Prevents the Development of Arthritis in Vaccinated Mice Challenged with Borrelia burgdorferi. Infect. Immun. 71: 3437-3442 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»