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Clinical and Diagnostic Laboratory Immunology, January 2003, p. 19-21, Vol. 10, No. 1
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.1.19-21.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Effect of Multiple Freeze-Thaw Cycles on Detection of Measles, Mumps, and Rubella Virus Antibodies

Norman A. Pinsky,1 Jeanne M. Huddleston,2 Robert M. Jacobson,1,3 Peter C. Wollan,4 and Gregory A. Poland1,2*

Mayo Vaccine Research Group,1 Department of Internal Medicine,2 Department of Pediatric and Adolescent Medicine, Mayo Clinic,3 Olmsted Medical Group, Rochester, Minnesota 559054

Received 21 August 2002/ Accepted 6 October 2002

We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4°C until assayed. Each assay measurement was done in replicates of five, and the mean value was reported. After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels and found no discernible detrimental effect on the ability to measure these antibodies by enzyme-linked immunoassays.

* Corresponding author. Mailing address: Mayo Vaccine Research Group, Mayo Clinic and Foundation, 611 C Guggenheim Building, 200 First St., SW, Rochester, MN 55905. Phone: (507) 284-4456. Fax: (507) 266-4716. E-mail: poland.gregory{at}mayo.edu.

Clinical and Diagnostic Laboratory Immunology, January 2003, p. 19-21, Vol. 10, No. 1
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.1.19-21.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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