This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Van Tassel, R. L. Articles by Wilkins, T. D. Search for Related Content PubMed PubMed Citation Articles by Van Tassel, R. L. Articles by Wilkins, T. D.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, 07 1994, 473-469, Vol 1, No. 4
Copyright © 1994 by the American Society for Microbiology. All rights reserved.

Production of antisera against the enterotoxin of Bacteroides fragilis and their use in a cytotoxicity neutralization assay of HT-29 cells

RL Van Tassel, DM Lyerly and TD Wilkins
Department of Biochemistry and Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061-0305, USA.

To study the enterotoxin of Bacteroides fragilis, the colon carcinoma cell line HT-29 was used in a standard cytotoxicity assay. We produced high-titer neutralizing antisera in rabbits and goats against both crude and purified toxin and developed a cytotoxicity neutralization assay for use in confirming enterotoxin activity in culture filtrates and stools. The neutralization titers of the antisera on the colon carcinoma cell line HT-29 ranged from 1,600 to 2,400. In an antibody screening enzyme-linked immunosorbent assay, titers ranged from 10(4) to 10(5). The antisera produced against the highly purified toxin also neutralized the enterotoxic activity of the toxin and were monospecific by immunoelectrophoresis.

This article has been cited by other articles:

• Wu, S., Dreyfus, L. A., Tzianabos, A. O., Hayashi, C., Sears, C. L. (2002). Diversity of the Metalloprotease Toxin Produced by Enterotoxigenic Bacteroides fragilis. Infect. Immun. 70: 2463-2471 [Abstract] [Full Text]  
• Sanfilippo, L, Baldwin, T J, Menozzi, M G, Borriello, S P, Mahida, Y R (1998). Heterogeneity in responses by primary adult human colonic epithelial cells to purified enterotoxin of Bacteroides fragilis. Gut 43: 651-655 [Abstract] [Full Text]